38.8 Active B12

38.8.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 11.6236886 1 0.0007 ***
Active.B12 0.6246244 1 0.4 ns
Study 1.2553100 1 0.3 ns
GA_samp 5.8859994 1 0.02 *
Active.B12:Study 1.2215246 1 0.3 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 0.2236814 1 0.6 ns
Active.B12 5.8074322 1 0.02 *
Study 6.2629796 1 0.01 *
GA_samp 273.7672393 1 <0.0001 ***
Active.B12:Study 3.3981099 1 0.07 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 21.2907592 1 <0.0001 ***
Active.B12 1.8527297 1 0.2 ns
Study 0.6170155 1 0.4 ns
GA_samp 67.9422285 1 <0.0001 ***
Active.B12:Study 1.4044250 1 0.2 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 2.149 2.149 0.1 ns
3 Study 1 Inf 2.603 2.603 0.1 ns
4 GA_samp 1 Inf 0.110 0.110 0.7 ns
2 Active.B12:Study 1 Inf 0.543 0.543 0.5 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 2.224 2.224 0.1 ns
3 Study 1 Inf 3.529 3.529 0.06 ns
4 GA_samp 1 Inf 0.005 0.005 0.9 ns
2 Active.B12:Study 1 Inf 0.674 0.674 0.4 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 2.640 2.640 0.1 ns
3 Study 1 Inf 6.084 6.084 0.01 *
4 GA_samp 1 Inf 0.720 0.720 0.4 ns
2 Active.B12:Study 1 Inf 1.825 1.825 0.2 ns

38.8.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 21.63 (5.61, 82.51) <0.0001
Active.B12 1.00 (0.99, 1.00) 0.66
StudySTOP 1.08 (0.68, 1.71) 0.75
GA_samp 1.10 (1.02, 1.19) 0.02
hPL (μg/ml)
Var Effect P
(Intercept) 1.64 (0.93, 2.89) 0.09
Active.B12 1.00 (1.00, 1.00) 0.10
StudySTOP 1.23 (1.00, 1.52) 0.05
GA_samp 1.32 (1.28, 1.37) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.15 (0.07, 0.32) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.50
StudySTOP 0.90 (0.67, 1.20) 0.48
GA_samp 1.22 (1.16, 1.28) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 39.49 (8.20, 190.17) <0.0001
Active.B12 1.00 (0.99, 1.00) 0.20
StudySTOP 0.52 (0.30, 0.92) 0.02
GA_samp 1.02 (0.92, 1.13) 0.69
HOMA-IR
Var Effect P
(Intercept) 14.10 (2.54, 78.34) 0.002
Active.B12 1.00 (0.99, 1.00) 0.20
StudySTOP 0.41 (0.22, 0.77) 0.005
GA_samp 1.00 (0.90, 1.11) 1.00
Glucose (mmol/L)
Var Effect P
(Intercept) 7.89 (5.95, 10.47) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.10
StudySTOP 0.75 (0.67, 0.85) <0.0001
GA_samp 0.99 (0.97, 1.02) 0.60

38.8.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 65.16846 16.81754 63.11382 38.91177 109.1425
STOP 70.25568 13.11414 23.90948 47.78970 103.2829
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 63.01763 5.794362 110.67270 52.52068 75.61254
STOP 77.78576 3.503714 42.71103 71.03001 85.18406
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.924916 0.2445933 123.7220 1.496869 2.475368
STOP 1.731100 0.0978188 129.1443 1.547991 1.935868
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 35.62240 8.801762 Inf 21.94846 57.81524
STOP 18.68387 1.919051 Inf 15.27702 22.85047
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 9.620282 2.6685058 Inf 5.585714 16.569023
STOP 3.965654 0.4371071 Inf 3.195160 4.921947
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.296768 0.3973147 Inf 5.564273 7.125691
STOP 4.743145 0.1192833 Inf 4.515022 4.982794

38.8.4 Comparisons

Effect for every 10 unit increase in Active B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Active.B1284 / Active.B1274 SCOPE 0.99 (0.94, 1.04) 0.7
PRL (ng/ml) Active.B1284 / Active.B1274 STOP 0.99 (0.94, 1.04) 0.7
hPL (μg/ml) Active.B1284 / Active.B1274 SCOPE 1.02 (1.00, 1.05) 0.1
hPL (μg/ml) Active.B1284 / Active.B1274 STOP 1.02 (1.00, 1.05) 0.1
GH2 (ng/ml) Active.B1284 / Active.B1274 SCOPE 1.01 (0.98, 1.05) 0.5
GH2 (ng/ml) Active.B1284 / Active.B1274 STOP 1.01 (0.98, 1.05) 0.5
Insulin (uU/ml) Active.B1284 / Active.B1274 SCOPE 0.96 (0.90, 1.02) 0.2
Insulin (uU/ml) Active.B1284 / Active.B1274 STOP 0.96 (0.90, 1.02) 0.2
HOMA-IR Active.B1284 / Active.B1274 SCOPE 0.95 (0.89, 1.03) 0.2
HOMA-IR Active.B1284 / Active.B1274 STOP 0.95 (0.89, 1.03) 0.2
Glucose (mmol/L) Active.B1284 / Active.B1274 SCOPE 0.98 (0.96, 1.00) 0.1
Glucose (mmol/L) Active.B1284 / Active.B1274 STOP 0.98 (0.96, 1.00) 0.1

38.8.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.8.6 Plots